Citrus Tissue Culture

Citrus tissue culture: stimulation of fruit explant cultures with orange juice.

In vitro growth of explant (juice vesicle or albedo tissues) cultures from citron (Citrus medica), lemon (C. limon), grapefruit (C. paradisi), sweet orange (C. sinensis), and mandarin (C. reticulata) fruits was stimulated by addition of orange juice (10% v/v optimum) to a basal medium containing Murashige and Skoog salts, 50 grams per liter sucrose, 100 milligrams per liter myo-inositol, 5 mill...

Tissue Culture

Dr. Willmer's second edition of this little book fulfills his stated purpose ... to correlate some of the numerous fragments of scattered data which have so far been obtained and to orientate them in such a way as to illustrate the more fundamental contributions which the method has made to physiological knowledge, and to show the manner in which a change of outlook has already been brought abo...

Adaptation of Horsesickness Virus to Tissue Culture

Tissue Culture Media

1. Human placental cord serum contains a factor or factors necessary for active growth of fibroblasts in tissue culture and a factor which prevents the appearance of a characteristic toxicity. 2. The growth-promoting factor(s) is stable to heating to 60 degrees C. for 30 minutes and is associated with the euglobulin fraction of the serum. 3. Extraction of dried cord serum with benzene, chlorofo...

Tissue culture screening.

A suspension of Ketawaspreparedby trypsinization of stock cultures in Blake bottles. After determining the cell count with a hemocytometer the suspension was diluted with medium to give a c@int of' 75,000 cells per 0. 9 ml. Using an automatic pi pette, 0. 9 m:1@. of the Hela suspension was delivered into each of the cups of the plastic panel and the test compound added in 0.1 ml. saline or in 0...